SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. It features a modular design that allows users to add or change detection cartridges according to their needs. The plate reader optics are tunable for. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. . Step 1: Turn on your microplate reader. Fluorescence Microplate Reader Comparison Chart. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. FI assay. 0 out of 5 $ 11,999. For a higher . The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Sensitive fluorescent quantitation of DNA with the Quant-iT. The CellTiter-Glo assay from Promega uses luciferase. The University of Rouen in Normandy uses the SpectraMax iD3 and. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Here, the CVs for each plate were less than 3, and the difference between mean. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Molecular Devices. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Molecular Devices SpectraMax M2 Multimode Plate Reader. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. Cell Cytotoxicity assay procedure. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Wide range of applications. 2 only: SpectraMax Mini Update. 0 out of 5 $ 6,499. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax® iD5 can be expanded to include bottom. Examples: “Press carefully to engage,” “ Do not press down. The price range of a reader starts at 2. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. . Examples: “Press carefully to engage,” “ Do not. Compact and tunable absorbance microplate readers DATA SHEET. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. SoftMax® Pro 7. Measuring platelet aggregation with a microplate reader. Example: [Drawer]. SpectraMax i3x Multi-Mode Detection Platform. Positions H21-K22 contained plate blanks of PBS only. Microplate Readers Biotek Epoch Microplate Reader. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Table 2 summarizes the results for the uniformity test. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. We would like to show you a description here but the site won’t allow us. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Table of Contents. The drawing shows a standard microplate of 15 mm height covered with a lid. Highly customizable low volume. Fill a clean plate with water. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. flexibility of monochromator-based systems. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. AlphaScreen. The SpectraMax 190 reader can be integrated with Molecular . 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. Plate chamber design of the SpectraMax category 1 microplate readers. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. We would like to show you a description here but the site won’t allow us. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Transcreener ® ADP. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. western blot membrane detection in a multimode microplate reader platform. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Microplate Readers. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Assays vary depending on. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. 313 reference gain check fail. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Five preferred detection modes. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Offer Ends In: 18 Days 19: 38: 21. limit of detection (LLD) for ATP was estimated for each microplate reader using the . 0 out of 5 $ 5,999. Users can perform spectral scans from 250 nm to 750. com. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Other technologies such as imaging. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Step 4: Incubate at room temperature for 1 hour, protected from light. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. Nucleic Acid and Protein Quantitation. The SpectraMax M2 and M2e are versatile and reliable. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The SpectraMax® iD3 Multi Mode Microplate . These lab workhorses are used in drug discovery, research, bioassay. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Detailed specifications of each model reader are reported in Section 7, “Specifications”. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Please refer to the Obsolete Microplate Reader Models for more information. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Fluorescence Microplate Readers. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. 0 out of 5 $ 2,999-20%. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. It can run samples based on pre-defined. Absorbance (optical density, OD) was read at 570 nm and 605. The instrument uses a paired monochromator and xenon flash. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. Molecular Devices Spectramax iD3 Microplate Reader. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 20 μL of Component A was added to each well of the microplate. A: Microplate Preparation. In . The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. , April 14, 2022 – Molecular Devices, LLC. flexibility of monochromator-based systems. 030 fmol Fluorescence Polarization (FP). The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Temperature control. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Download PDF versions of the SpectraMax Microplate Reader User Guides. Self Service Available. Figure 6: Transcreener ADP2 FI assay principle. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. SpectraMax Microplate Readers. If it looks fine, then proceed. Request a Quote!Introduction. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. Published Dec 16, 2020 | Updated Dec 16, 2020. Download PDF. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The temperature in the plate chamber is ambient and isothermal. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. . For 40 years, we have partnered with scientists to help them achieve landmark discoveries. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Download PDF. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. Wide range of applications The VersaMax reader covers. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. . 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. Step 2: Launch SoftMax Pro Software. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. 1) Select the correct plate and the wells that you want to measure. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Figure 2-23: Bottle holder To install the bottle holder: 1. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. begin reading microplates with seven mouse clicks. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. SpectraMax Glo Steady-Luc Reporter Assay Kit. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. 3) This warranty. 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Turn the reader off by pulling the power cord. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. 470 nm). SpectraMax Mini Plug-In for SoftMax Pro 7. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. Example: [Drawer]. 380 nm/Exc. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Our specialization servicing. Path Check Pathlength Measurement every Gemini EM Reader. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. e. Step 2: Launch SoftMax Pro Software. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Find our technical support information along with contact details. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. B: Selection of Excitation Wavelength. We would like to show you a description here but the site won’t allow us. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. com 4. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. This unlocks the transport slide. 003 fmol 0. 1. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. 76358-624. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 314 low lamp level warning. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Dual-Glo luciferase assay system (Promega Cat. 6 for further experiment, so I'm confused which i would choose for OD value determination. 7°C (gradient heating design). Italic and boldface type are used for emphasis. Multimode Microplate Reader. Or, with the reader specifications noted from the Instrument Description section of your reader's. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Temperature control and shaking come as standard. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax Plus: SpectraMax Plus. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. 304 signal level saturation. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Data exemplifying linear ranges from the three different kits are presented in Figure 2. It also integrates with the. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The dual monochromator optics allow. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. Category 2 and 3 microplate readers use gradient heating as well. Temperature control. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. Instrument settings used with the SpectraMax M5 Microplate Reader. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Other features and benefits include: Eliminate changing out filters. With optimized reagents, option to operate the. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. No accessories are needed and SoftMax® Pro Software. Table of Contents. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. Molecular Devices: Molecular Devices. Align the two slots on the bottle holder with the two pegs on the rail. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Nuclear Receptor. 997 (n = 3). It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. In addition, the SpectraMax iD5 can be expanded to. Add to Cart. Selection of Water Constant in PathCheck settings. 2 software: see SoftMax® Pro 7. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. flexibility of monochromator-based systems. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Bookings are free, but you will need to use iLab to book. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. \ Figure 3. SpectraDrop High-Throughput Screening (HTS) Kit. 315 can't find zero order. In addition, the SpectraMax® iD5 can be expanded to include bottom. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Products : General, Microplate Readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The dual monochromator optics allow the. We would like to show you a description here but the site won’t allow us. The plate reader can measure multiple readouts, including UV and visible absorbance. SAN JOSE, CA — Molecular Devices, LLC. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The following document is intended to demonstrate setup of this instrument. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Time-. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. 5 12. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. 58% for the detection module and 2. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Figure 1.